Abstract
To understand biological significance of a 34kDa ribonucleoprotein (p34) in the chloroplast gene expression, effects of DNAs and RNAs on the phosphorylation of p34 by CK-II were analyzed biochemically. CK-II catalyzed phosphorylation of p34 was stimulated about 12-fold by dsDNA and 4.2-fold by poly (rG), respectively. The analysis of CD spectra revealed that DNA and RNA induced conformational change of p34. These results suggest that the conformational modification of p34 by nucleic acids results in enhancement of the affinity between the protein and CK-II. Based on the experimental results and findings of previous reports, a possible model for post-transcriptional regulation through phosphorylation of p34 by CK-II in chloroplast gene expression is proposed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
