Abstract
Increased nitrogen runoff from commercial fertilizer use has raised issues regarding hypoxia in freshwater habitats. One species noted as a water indicator is planarians, known for their pluripotent stem cell regeneration. This study tested the effects of various nitrate concentrations on planarian specimens with ablated heads, to ascertain the effects of the nitrate on regeneration and survivability. The tests found that there is a negative correlation between nitrate concentration and planarian survival rates, proving that nitrogen runoff can indeed adversely affect similar organisms in a freshwater habitat.
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