The effects of NF-κB decoy oligodeoxynucleotides on expression of co-stimulatory molecules in Kupffer cells

Abstract

Objective To investigate the inhibitory effects of NF-κB decoy oligodeoxynucleotides (ODN) on the expression of co-stimulatory molecules in Kupffer cells (KCs). Methods KCs were isolated by in situ collagenase perfusion and randomly divided into three groups: control group, LPS stimulation group (1 mg/L LPS), and NF-κB decoy ODN group, in which KCs were transfected with NF-κB decoy ODN (4 μg/1×105 KCs) before LPS stimulation. After 8 h of LPS stimulation, KCs were collected and CD80, CD40,and CD54 mRNA expression was assayed by reverse transcription-polymerase chain reaction. Results LPS stimulation significantly increased the expression of co-stimulatory molecules (CD80,CD40,and CD54) in KCs by 2.53,2.51 ,and 2.74 fold respectively over that of the control group (P 0.05). Conclusion NF-κB decoy ODN can. efficiently inhibit the expression of co-stimulatory molecules in KCs,which provides reliable experimental data for the clinical application of NF-κB decoy ODN to inhibit acute rejection after liver transplantation in vivo. Key words: NF-kappa B; Oligodeoxynucleotides; Kupffer cells; Antigens, CD80; Antigens, CD40