The effects of new herbal formula (KBMSI-2) on penile erection and expression of nitric oxide synthase isoforms in streptozotocin-induced diabetic rat model.

Abstract

To investigate the effects of new herbal formula (KBMSI-2) on erectile dysfunction in streptozotocin (STZ)-induced diabetic rat model. Twenty four Sprague-Dawley male rats were randomly divided into three groups; control (n=8), diabetes model (n=8), diabetes + KBMSI-2 200 mg/kg treatment (n=8) groups. The diabetes induced groups received a single intraperitoneal injection of STZ. Distilled water was administered in the control and model groups. To investigate the penile erection, intracavernosal pressure (ICP) and intracavernosal pressure/mean arterial pressure (ICP/MAP) were recorded in all groups. Serial sections of the penis were used to perform Masson's trichrome stain. The expression of neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS) and cyclic guanosine monophosphate (cGMP) concentration in the isolated corpus cavernosum were analyzed by Western blotting. Peak ICP/MAP ratio was increased in the KBMSI-2 treatment group compared with the model group (P<0.05). Masson's trichrome staining confirmed that the smooth muscle component was increased in the KBMSI-2 treatment group compared with the model group (P<0.05). The nNOS, eNOS and cGMP expression of KBMSI-2 200 mg/kg treatment group was increased compared with the model group (P<0.05). This study showed that herbal formula of KBMSI-2 improved the erectile function by preserving the smooth muscle content and inhibiting the fibrosis of the corpus cavernosum in STZ-induced diabetic rat model.