Abstract
The investigation of the effects of mutastein and dextranase on the crude GTF derived fromS. mutans B13, and liveS. mutans B13, were made, and the following results were ohtained. 1) The amount of insoluble glucans formed on the surface of the wall of a test tube in the presence of mutastein or dextranase decreased with the increase in the concentration of mutastein or dextranase. The amount of synthesized water-soluble glucans and insoluble non-adherent glucans increased with the increase in the concentration of mutastein or dextranase up to a certain point. 2) The formation of plaques in the presence of live bacteria was significantly suppressed when mutastein or dextranase was added. 3) By observation with the observation with a scaning electron microscpe (SEM), it was found that the addition of mutastein caused a change in the form of the of bacterial cells. It was also observed that the amount of glucans decreased with the increase in the concentration of mutastein or dextranase added.
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