The effects of miRNA-27a-3p on human epidermal melanocytes.

Abstract

To characterize the effects of miRNA-27a-3p on the biological properties of human epidermal melanocytes (MCs). MCs were obtained from human foreskins and transfected with miRNA-27a-3p mimic (induces the overexpression of miRNA-27a-3p), mimic-NC (the negative control group), miRNA-27a-3p inhibitor, or inhibitor-NC. After transfection, the proliferation of MCs in each group was evaluated by cell counting kit-8 (CCK-8) at 1, 3, 5, and 7 days. Twenty-four hours later, the MCs were transferred onto a living cell imaging platform and cultured for another 12h to detect their trajectories and velocities. On days 3, 4, and 5 after transfection, the expression of melanogenesis-related mRNAs, protein levels, and melanin contents were measured using reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and NaOH solubilization, respectively. The RT-PCR results showed that miRNA-27a-3p was successfully transfected into MCs. The proliferation of MCs was restrained by miRNA-27a-3p. There were no significant differences in the movement trajectories of MCs in the four transfected groups, but the cell movement velocity in the mimic group was slightly lower; that is, the overexpression of miRNA-27a-3p inhibited the speed of MCs. The expression levels of melanogenesis-related mRNAs and proteins were decreased in the mimic group and were increased in the inhibitor group. Melanin content in the mimic group was lower than that in the other three groups. Overexpression of miRNA-27a-3p inhibits the expression of melanogenesis-related mRNAs and proteins, reduces the melanin content of human epidermal MCs, and slightly impacts their movement speed.