Abstract
MiRNAs are known to play important roles in cancer cell development. However, the pattern and biological role of miR-183 in endometrial cancer (EC) have not been completely unexplored. Here, we found that miR-183 was upregulated in endometrial cancer cells. The purpose of the study was to evaluate the function of miR-183 in the endometrial cancer cell line and the mechanisms regulating its direct target protein in these processes. The mRNA and protein expressions were analyzed by quantitative RT-PCR and western blotting, respectively. The experiments about MTT assay, colony formation assay and transwell assay showed that miR-183 can positively regulate cell proliferation, migration and invasion in vitro. Furthermore, the in vivo experiments indicated that knockdown of miR-183 significantly attenuated EC cells growth. Mechanistically, luciferase reporter assay and western blotting assay was conducted to confirm target associations. The data analysis revealed that MMP-9 as a direct target of miR-183 in EC and there was a negatively relationship between miR-183 and MMP-9 expression in EC cells. Taken together, our results demonstrated that miR-183 plays a critical role in EC tumorigenesis and metastasis by suppressing MMP-9 expression, which may be an attractive therapeutic target for the treatment of endometrial cancer.
Published Version
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