Abstract

Objective To investigate the mechanisms and the effects of magnesium Valproate on the expressions of the kinin B1 and B2 receptors in the hippocampus of the juvenile rats submitted to pilocarpine model of epilepsy. Methods 35 healthy Wistar juvenile rats were randomly divided into six groups, that is the model groups: Ⅰgroup, Ⅱgroup, Ⅲgroup, and intraperitoneal injection of saline water control groups : Ⅰa group, Ⅱa group, Ⅲa group , after succession of 15 rats to kindle to establish the model of epilepsy by pilocarpine. To collect hippocampus tissue after the rats were to put to death, and to compared the expression levels of kinin B1 and B2 receptor mRNA by RT-PCR and western blot in the hippocampus of rats. Results By treated with magnesium valproate, kinin B1 receptor mRNA (0.38±0.051)and protein expressions(0.58±0.057)decreased and kinin B2 receptor mRNA (0.48±0.056)and protein expressions(0.48±0.044)increased in Ⅰ group, compared with that(0.76±0.068, 0.89±0.034; 0.28±0.034, 0.32±0.039) of Ⅰa group(P<0.05). Compared with control group, there were more significant upregulation of kinin B1 receptor mRNA and protein expressions (P<0.05) in the Ⅰ and the Ⅱ groups and there were no alteration in Ⅲ group.The expressional levels of B2 receptor mRNA and protein were upregulated in the Ⅰ, Ⅱ and Ⅲ groups. Conclusion The kinin B1 and B2 receptor may play a role in the onset and maintenance of epilepsy. The magnesium valproate increased the expressional levels of kinin B2 receptor, and decreased the expressional levels of kinin B1 receptor. Key words: Kinin B receptor; Magnesium valproate; Epilepsy model; Pilocarpine

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