The effects of low level laser therapy on Staphylococcus aureus infected third-degree burns in diabetic rats.

Reza Ranjbar,Mohammad Ashrafzadeh Takhtfooladi

doi:10.1590/s0102-865020160040000005

Reza Ranjbar, Mohammad Ashrafzadeh Takhtfooladi

Open Access

https://doi.org/10.1590/s0102-865020160040000005

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Abstract

To characterize the effects of low-level laser (LLL) on third-degree burn wounds which were infected with Staphylococcus aureus (S. aureus) in diabetic rats. Thirty streptozotocin-induced diabetic rats were divided into two groups: the control and the LLL groups. Third-degree burns were induced using a heated metal rod, and then, were contaminated with S. aureus. The wounds in the LLL group were irradiated with a LLL (685nm) daily for five consecutive days, starting three days after the induction. The wound area was measured at 3, 5, 8, 14 and 21 days after burning. At the end of trial, the skin samples were harvested. Reduction in wound areas in the LLL and control groups were significantly different only on the 21st day (p<0.05). The mean bacterial numbers in the LLL group were significantly lower (p<0.05) than those in the control group. The number of macrophages, new blood vessels, fibroblast, and elevated collagen deposition in the LLL group significantly increased compared to the control group (p<0.05). The mean breaking strength of scars in the control group was significantly lower (p<0.05) than that of the LLL group. The low-level laser improved the healing of S. aureus third-degree burn infections in diabetic rats.

Highlights

Skin wound healing, especially in diabetic patients, is one of the most important issues in medical sciences and has always been of interest to researchers and clinicians who have put much effort into accelerating the wound healing process, preventing infections, and increasing the tensile strength of the healing tissue after recovery[1]
The use of Level Laser (LLL) therapy as a photonic treatment to stimulate or accelerate the healing of the wounds started by Mester in 1970, and further studies have been conducted on its effects on wound healing since then[7,8,9,10,11]
The most important mechanism proposed in this field is the effect of LLL on shortening the tissue inflammation and accelerating the proliferation phase, anti-bacterial effect, effect on mitochondrial function of cells, increasing the blood supply to tissues and cell membrane potential changes which eventually can accelerate wound healing process[12,13,14]

Summary

Introduction

Especially in diabetic patients, is one of the most important issues in medical sciences and has always been of interest to researchers and clinicians who have put much effort into accelerating the wound healing process, preventing infections, and increasing the tensile strength of the healing tissue after recovery[1]. The delay in the healing of wound infections in diabetic patients is a problem caused by neuropathy and impaired tissue blood flow[2]. That’s why new methods must be used to defeat infections caused by these bacteria One of these methods can be the use of Low Level Laser (LLL). The use of LLL therapy as a photonic treatment to stimulate or accelerate the healing of the wounds started by Mester in 1970, and further studies have been conducted on its effects on wound healing since [7,8,9,10,11]. The most important mechanism proposed in this field is the effect of LLL on shortening the tissue inflammation and accelerating the proliferation phase, anti-bacterial effect, effect on mitochondrial function of cells, increasing the blood supply to tissues and cell membrane potential changes which eventually can accelerate wound healing process[12,13,14]

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