Abstract
The influence of various ions and of dopamine and somatostatin on the in vitro activity of rainbow trout prolactin (PRL) cells was investigated. There was a positive correlation between medium Ca 2+ concentration and both PRL synthesis and release up to 1.8 m M Ca 2+, above which no further increase occurred. Even with no Ca 2+ in the medium, there was still PRL secretion during the incubation. Replacement of Ca 2+ with Ba 2+ in the medium did not elevate either total PRL levels or PRL release above that in Ca 2+-free medium. Neither elevated Mg 2+ nor increased medium K + had any effect on PRL synthesis or release. Dopamine inhibited PRL release but not synthesis, as did the D 2 receptor agonist, apomorphine. However, the D 2 receptor antagonist, (+)-butaclamol was unable to prevent the action of dopamine on PRL release. Somatostatin inhibited both PRL synthesis and release in normal Ca 2+ medium, but release only in reduced Ca 2+ medium. Thus, Ca 2+, dopamine, and somatostatin may all have roles in regulating prolactin secretion in this fish. In addition, oPRL reduced trout PRL release, indicating a possible negative feedback mechanism for trout PRL secretion.
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