Abstract
Alcohol consumption is a major health issue and associated with human cancers, such as liver and breast cancers. Alcohol was classed as carcinogen to human by IARC. We have performed in vivo and in vitro studies which demonstrate that diluted ethanol promotes cell proliferation and transformation and tumor formation. Consumption of liquor spirits (white wines) is a popular behavior. However, it is unclear whether liquor spirits affect cellular phenotypes of human cancers. At present study, we used diluted ethanol and liquor spirits (Sample #1 and Sample #2) to determine the changes in RNA polymerase III-dependent gene (Pol III gene) transcription, cell growth and colony formation in the different human cancer lines. The results indicate that low concentration of ethanol increases RNA Pol III gene transcription and rate of cell growth. However, both liquor spirits (Sample #1 and Sample #2) inhibit the activity of RNA Pol III genes and repress cell proliferation of the cancer lines, compared to diluted ethanol. The liquor spirits reduce the rate of colony formation of human breast cancer cells and esophageal carcinoma cells. The inhibitions of the liquor spirits to RNA Pol III genes, cell growth and colony formation are in a dose-dependent manner. These new findings suggest that the liquor spirits contain some active components to repress Pol III gene transcription and cell growth caused by ethanol in different human cancer cells.
Highlights
Numerous studies have indicated that alcohol intake is associated with human cancers in different organs, such as breast, liver, stomach, pancreas, oral cavity, pharynx, esophagus, larynx, colon and ovary [1]-[8]
The results indicate that low concentration of ethanol increases RNA Pol III gene transcription and rate of cell growth
The results reveal that both liquor spirits (Sample #1 and Sample #2) inhibit Pol III gene activity and decrease the rates of cell proliferation of these cancer lines, compared to diluted ethanol treatment
Summary
Numerous studies have indicated that alcohol intake is associated with human cancers in different organs, such as breast, liver, stomach, pancreas, oral cavity, pharynx, esophagus, larynx, colon and ovary [1]-[8]. To investigate whether the liquor spirits are able to inhibit cell growth of human cancers, we have utilized two liquor spirits of China, a Nong-Xiang liquor (Sample #1) and a Jiang-Xiang liquor (Sample #2), to determine whether the two liquor spirits affect RNA Pol III gene transcription and cellular phenotypes of different human cancer lines. The results reveal that both liquor spirits (Sample #1 and Sample #2) inhibit Pol III gene activity and decrease the rates of cell proliferation of these cancer lines, compared to diluted ethanol treatment. The two liquor spirits inhibit colony formation of human breast cancer and esophageal carcinoma lines These studies suggest that the liquor spirits may contain some bioactive components, which are of capacity of repressing ethanol-induced Pol III gene transcription and decreasing ethanol-enhanced the rates of cell growth and colony formation in different human cancer lines
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