Abstract

Some effects of light on morphogenesis in Sclerotium rolfsii Sacc. were studied. Physiological competence to visible light developed during the first 120 h after inoculation, with an optimum sensitivity phase between 84 and 96 h that coincided with the leading hyphae reaching the edge of the Petri dish. Although sclerotial initials were produced in dark-grown cultures, light was necessary for the continuation of the developmental and maturation phases of sclerotial morphogenesis. Tyrosinase activity (o-diphenol: oxygen oxidoreductase, EC 1.10.3.1) was detected during sclerotial formation and the pH and temperature optima for his polyphenol oxidase in vitro were about 6.0 and 45 degrees C respectively. The enzyme was inhibited by cysteine. Similar activity levels of tyrosinase were obtained in blue and "white" light-grown cultures but in red light activity was comparable with that of dark-grown cultures. Laccase activity was not detected at any stage of development.

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