Abstract
By comparing (32)P-orthophosphate incorporation into nucleic acid extracts of sterile and non-sterile radish leaf discs, it was shown that contaminating bacteria cause a 2-4 fold increase in the rate of precursor incorporation and alter the pattern of label distribution after fractionation of the extracts by sedimentation through sucrose gradients or chromatography on MAK(1) columns. Using sterile senescing radish leaf discs, a stimulation of (32)P-orthophosphate incorporation into various fractions of nucleic acid was observed as a result of kinetin treatment.
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