Abstract
Objective To investigate the effects of intro-oral injection of parathyroid hormone (PTH) on tooth extraction wound healing in hyperglycemic rats.Methodology 60 male Sprague-Dawley rats were randomly divided into the normal group (n=30) and DM group (n=30). Type 1 diabetes mellitus (DM) was induced by streptozotocin. After extracting the left first molar of all rats, each group was further divided into 3 subgroups (n=10 per subgroup), receiving the administration of intermittent PTH, continuous PTH and saline (control), respectively. The intermittent-PTH group received intra-oral injection of PTH three times per week for two weeks. A thermosensitive controlled-release hydrogel was synthesized for continuous-PTH administration. The serum chemistry was determined to evaluate the systemic condition. All animals were sacrificed after 14 days. Micro-computed tomography (Micro-CT) and histological analyses were used to evaluate the healing of extraction sockets.Results The level of serum glucose in the DM groups was significantly higher than that in the non-DM groups (p<0.05); the level of serum calcium was similar in all groups (p>0.05). Micro-CT analysis showed that the DM group had a significantly lower alveolar bone trabecular number (Tb.N) and higher trabecular separation (Tb.Sp) than the normal group (p<0.05). The histological analyses showed that no significant difference in the amount of new bone (hard tissue) formation was found between the PTH and non-PTH groups (p>0.05).Conclusions Bone formation in the extraction socket of the type 1 diabetic rats was reduced. PTH did not improve the healing of hard and soft tissues. The different PTH administration regimes (continuous vs. intermittent) had similar effect on tissue healing. These results demonstrated that the metabolic characteristics of the hyperglycemic rats produced a condition that was unable to respond to PTH treatment.
Highlights
The different parathyroid hormone (PTH) administration regimes had similar effect on tissue healing. These results demonstrated that the metabolic characteristics of the hyperglycemic rats produced a condition that was unable to respond to PTH treatment
The macromolecular weight estimated from 1H Nuclear Magnetic Resonance (1H NMR) spectrum was 3246, which was consistent with theoretical estimation, indicating that the PECE gel was synthesized successfully
These findings suggest that hyperglycemic rats failed to benefit from the anabolic treatment of PTH on the healing of extraction socket in the high glucose condition
Summary
Diabetes mellitus (DM) has an increasingly higher occurrence of 463 million adults worldwide. People with DM are more likely to suffer from earlier detrimental oral status and are prone to numerous oral diseases, including oral infection, periodontitis and difficulties in wound healing after tooth extraction. It has been found that the prevalence of tooth extractions in DM patients is 1.88 times higher than the general population.. The aim of this study was to investigate and compare the effects of local administration (intro-oral injection) of intermittentand continuous-PTH on the healing of tooth extraction sockets in hyperglycemic rats. The intermittent intra-oral injection of PTH (1-34) (Chinese Peptide, Hangzhou, China) was administered at a dose of 80 μg/kg three times a week for 14 days. . The intermittent intra-oral injection of PTH (1-34) (Chinese Peptide, Hangzhou, China) was administered at a dose of 80 μg/kg three times a week for 14 days.17, 23 It was dissolved in normal saline solution, and the needle was placed in the buccal vestibule next to the tooth extraction site.
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