Abstract
AbstractThe results of a theoretical treatment are given for the effect of intraparticle diffusion on the Michaelis‐Menten kinetic behaviour of enzymes entrapped in spherical hydrogel particles. It is shown that curvature in Lineweaver‐Burk plots may be expected, and that the influence of particle size on the activity of entrapped enzymes may be predicted. The effectiveness factor is discussed and analogies with heterogeneous catalysis noted. Experimental results are presented for the hydrolyses of model substrates by gel entrapped trypsin, β‐galactosidase, glucose oxidase and urease which are consistent with the theoretical treatment.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
