The effects of innervation zone on electromyographic amplitude and mean power frequency during incremental cycle ergometry

Abstract

The purpose of this study was to examine the effects of electrode placements over the innervation zone (IZ), as well as proximal and distal to the IZ, on the patterns for the absolute and normalized electromyographic (EMG) amplitude and mean power frequency (MPF) versus power output relationships during incremental cycle ergometry. Fifteen men [mean ± S.D. age = 24.3 ± 2.4 years; V ˙ O 2 max = 47.3 ± 4.9 ml k g − 1 mi n − 1 ] performed incremental cycle ergometry tests to exhaustion. Surface EMG signals were recorded simultaneously from bipolar electrode arrangements placed on the vastus lateralis (VL) muscle over the IZ, as well as proximal and distal to the IZ. Polynomial regression analyses were used to describe the relationships for absolute and normalized EMG amplitude (μVrms and %max) and MPF (Hz and %max) versus power output (%max) for each subject at the three electrode placement sites. In addition, separate one-way repeated measures ANOVAs were used to examine mean differences between the three sites for absolute and normalized EMG amplitude and MPF at power outputs of 80, 110, 140, and 170 W. The results of the polynomial regression analyses revealed that the best fit model for each site for the absolute and normalized EMG amplitude versus power output relationship was linear for 11 subjects and quadratic for 2 subjects. The remaining two subjects exhibited both linear and quadratic patterns that were site-dependent. For EMG MPF, 10 subjects exhibited significant relationships (linear and/or quadratic) across power outputs for at least one site. In addition, there were significant ( P < 0.05) mean differences between the electrode placement sites for absolute EMG amplitude, but not absolute EMG MPF at 80, 110, 140, and 170 W. There were no significant ( P > 0.05) mean differences, however, between the three sites for normalized EMG amplitude or MPF at 80, 110, 140, and 170 W. These findings indicated that the placement of bipolar electrodes over the IZ, as well as proximal and distal to the IZ, had no effect on the pattern of the normalized EMG amplitude versus power output relationship or the mean normalized EMG amplitude and MPF values. Thus, during cycle ergometry, normalized EMG amplitude values (but not absolute values) can be compared between studies that have utilized various electrode placement sites on the VL.