The Effects of Immobilization Conditions and Materials on the Desulfurization Performance of Immobilized UP-3

Abstract

Immobilization of the bacterium Agrobacterium tumefaciens UP-3, which was isolated from soil and sewage in the Shengli Oilfield, was studied in this paper. The suitable culture condition of growth cells was determined. The effects of immobilization conditions and materials on the desulfurization performance of immobilized cells were investigated. The results showed that the immobilized cells with mixture of sodium alginate (SA) and polyvinyl alcohol (PVA) as the immobilized carrier had good biodesulfurization characteristics; The optimum operation immobilization conditions are 4° C, the concentration of SA being 3% (w), the concentration of PVA and SA being 7% (w), and the ratio of carriers (ml) and cells (g) being 20. When DBT addition is 2.7 mM, the DBT degradation rate of immbobilized cells is above 60%.