Abstract
Incorporation of 14C from U-14C-glucose into fatty acids and CO2 by epididymal fat was studied in vitro. Simultaneous measurements of glycerol and free fatty acid (FFA) production were also made. Induction of diabetes with streptozotocin (50 mg/kg iv.) markedly inhibited fatty acid synthesis and increased glycerol and FFA production. Total adipose tissue mass was grossly reduced. Hypophysectomy of the diabetic animals reversed these changes. Oxidation of glucose to 14CO2 was only slightly affected by any of the procedures. Three hrs. after administration, growth hormone (100/µg/rat) inhibited glucose oxidation and fatty acid synthesis in both hypophysectomized and hypophysectomized-diabetic animals. Similarly, when added in vitro to adipose tissue of hypophysectomized-diabetic rats, growth hormone (1 µg/ml) increased the oxidation of glucose and the incorporation of its carbons into fatty acids. These observations are consonant with the suggestion that both the early, insulin-like and the late, anti-insulin-like effects of growth hormone may be independent of endogenous insulin. The data also indicate that in isolated adipose tissue as in whole animals, the metabolic effects of insulin deficiency can be reversed by hypophysectomy.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
