Abstract
The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.
Highlights
University of Zagreb School of Dental Medicine1, Institute for Medical Research and Occupational Health2, Zagreb, Croatia
Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility
In this study we tested three preparations used in dental medicine: [1] calcium hydroxide preparation (ApexCal®, Ivoclar Vivadent, Liechtenstein), which is composed of calcium hydroxide, bismuth carbonate, polyethylene glycol, glycerine, and water; [2] dentin adhesive (Excite®, Ivoclar Vivadent, Liechtenstein), which is composed of phosphonic acid acrylate, hydroxyethyl methacrylate, dimethacrylate, highly dispersed silica, ethanol, catalysts, stabilizers, and fluoride; and [3] hyaluronic acid gingival gel
Summary
THE EFFECTS OF HYALURONIC ACID, CALCIUM HYDROXIDE, AND DENTIN ADHESIVE ON RAT ODONTOBLASTS AND FIBROBLASTS. The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. HA is synthetised on the cytoplasmatic surface of plasma membranes [25, 26] and is common in humans and other vertebrates It is the major component of the inner-cell cement, of the capillary wall, and of the extracellular matrix of the connective tissue [27]. The aim of this study was to investigate the effects of HA on the proliferation of odontoblasts and fibroblasts and to assess its potential as a material for direct pulp capping in humans. We assessed the effects of calcium hydroxide and of one of commonly used dentin adhesives
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