Abstract
Fractions of normal human serum were obtained by ammonium sulfate or acetic acid precipitation, followed by DEAE-cellulose and molecular sieve chromatography. These fractions were shown to inhibit or enhance the human lymphocyte response to phytohemagglutinin P and concanavalin A as measured by 3H-thymidine incorporation. Inhibition was reproducibly observed when certain of the serum fractions were added to the lymphocyte cultures, even up to 24 hr after the mitogen was added. The main identifiable components of the mitogenesis inhibitory serum fractions were carbohydrate-containing α-glycoproteins. It is suggested that this type of inhibition results from an interaction of the mitogen and the glycoprotein which reduces the amount of mitogen available to stimulate blast transformation.
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