The effects of human plasma protein solution 5% on blood coagulation and chemistry, an experimental in-vitro study

Abstract

Objective: The effect of different fluids used for resuscitation was studied in the literature in vitro and the results were different and not consistent. This study aims to study the in-vitro effect on coagulation of a newly used Human Plasma Protein (PP) solution 5% solution as a primary end point and its effect on blood SID and PH as secondary end points. Methods: In an experimental in-vitro study, different dilutions 10, 30 and 50% of PP 5% solution and fresh blood samples collected from volunteers, in which one cc of PP 5% is mixed to 9 cc of blood (10% dilution), 3 cc of PP 5% to 7 cc of Blood (30% dilution), and 2.5 cc of PP 5% to 2.5 cc of blood (50% dilution). Blood samples were analyzed for slandered Thrombo-Elastogram (TEG) and functional Fibrinogen level. TEG measurements included: r time, K time, α angle, ma (Maximum Amplitude), CI (Coagulation index), and LY 30 (lysis after 30 min of MA). Results: Reference figure below. Conclusion: The changes in coagulation induced by 30 and 50% dilution were significant. Changes in fibrinogen with extreme dilution of 50% do not indicate fibrinogen transfusion and haemoglobin level stay within the acceptable range and below transfusion trigger for healthy patients.