The effects of human atrial 28-amino acid peptide on systemic and renal hemodynamics in anesthetized rats.

Abstract

The effects of synthetic human atrial 28-amino acid peptide (alpha-human atrial polypeptide) on systemic and renal hemodynamics were examined in two separate groups of Inactin-anesthetized rats. First, mean arterial pressure, heart rate, and cardiac output were measured before and during infusion of the peptide at rates of 0.04-0.67 microgram/kg per min. Cardiac output was determined by the dye-dilution method with a microcuvette inserted into a carotid-femoral arterial shunt. After 10 minutes of infusion, mean arterial pressure, cardiac output, and total peripheral resistance were reduced in a dose-dependent manner by 21% (P less than 0.001), 9% (P less than 0.05), and 11% (P less than 0.05), respectively, with 0.67 microgram/kg per min of alpha-human atrial natriuretic polypeptide. Despite the marked fall in blood pressure, the heart rate did not change. Second, urine volume, urinary sodium excretion, glomerular filtration rate ([3H]inulin clearance) and renal blood flow ([14C]p-aminohippuric acid sodium clearance and hematocrit) were measured. Increases in urine volume, urinary sodium excretion, filtration fraction, and fractional sodium excretion and a decrease in renal vascular resistance were dose dependent: +490% (P less than 0.01), +1340% (P less than 0.01), +19% (P less than 0.05), +1160% (P less than 0.01), and -18% (P less than 0.05), respectively, with 0.67 microgram/kg per min of alpha-human atrial natriuretic polypeptide. The glomerular filtration rate increased with 0.33 and 0.67 microgram/kg per min of alpha-human atrial natriuretic polypeptide (both P less than 0.05), whereas renal blood flow did not change.(ABSTRACT TRUNCATED AT 250 WORDS)