The effects of high-density polyethylene and polypropylene microplastics on the soil and earthworm Metaphire guillelmi gut microbiota

Abstract

As an emerging pollutant in terrestrial ecosystem, studies on the effects of microplastics on the gut microbiota of terrestrial organisms are relatively little even though gut microbiota is closely related to host health, metabolism and immunity as well as soil decomposition processes. In this study, earthworms Metaphire guillelmi were exposed to soil amended with 0.25% (w/w) high-density polyethylene (HDPE, 25 μm) or polypropylene (PP, 13 μm) microplastics for 28 d. The ingestion of HDPE and PP microplastics by M. guillelmi was clearly demonstrated by Nile Red fluorescence staining method. There were significant differences for the microbiota between the M. guillelmi gut and the surrounding soil, which may result from the influence of specific conditions in the gut habitat. HDPE and PP microplastics exposure did not induce gut microbiota dysbiosis in M. guillelmi. However, PP microplastics exposure significantly reduced bacterial diversity and altered bacterial community structure in the soil. Specifically, the relative abundance of Aeromonadaceae and Pseudomonadaceae significantly increased while the relative abundance of Nitrososphaeraceae and two unidentified families affiliated with Proteobacteria significantly decreased. This study broadens our understanding of the ecotoxicity of microplastics on the soil and gut microbiota of terrestrial organisms.