Abstract
Lipases are mainly applied in the food, abluent and medicine industries. Through the high production costs of lipase enzymes for industrial applications, cheap and eco-friendly enzyme production has gained great importance in recent years. Yeasts can produce lipase enzyme and grow in acidic media. In the present study, the act of Cu2+, Ni2+ and molasses concentrations on the enzyme activity of Candida yeasts were investigated in a batch system. The maximum enzyme activities of microorganisms were determined at pH:4. Lipase enzyme activity was investigated changing metal ion and molasses sucrose concentrations by 25-250 mg/L and 1-20 g/L respectively. When molasses sucrose concentration was increased, the enzyme activity of all yeasts increased to 10 g/L, and the lipase enzyme activity decreased at the higher molasses concentrations. Enzyme activity of yeasts decreased with increasing both metal ion concentrations at constant molasses sucrose concentrations. Ni2+ cations were more inhibited to enzyme activity of all yeasts than Cu2+. Among the yeasts, Candida membranefeciens (936.96 U/L) showed the highest enzyme activity in media containing a constant molasses concentration of 10 g/L.
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