Abstract
The effects of heat-shock pretreatment conditions on the efficiency of dark hydrogen fermentation from glucose were investigated. Anaerobic sludge was pretreated at different temperatures of 70°C, 80°C, and 90°C for different time durations including 7.5, 15, 22.5, 30, 37.5, and 45 min. All pretreatments suppressed the methanogens and no detectable methane was produced after pretreatments. Pretreatments at 90°C led to the higher hydrogen contents than 70°C and 80°C. The pretreatment at 90°C for 37.5 min led to the highest biogas yield, hydrogen yield, and hydrogen production rate of 4.31 mol/mol-glucose, 3.15 mol/mol-glucose, and 70.2 mL/h.g-VS, respectively.
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