The Effects of Ginseng Java Roots (Talinum Paniculatum) extract on Malondialdehyde (MDA) levels in Male White Sprague Dawley Rats with Forced Swimming Test Model

Abstract

Intoduction: Cell and tissue damage can be caused by chemical species which known as free radical. Free radical is a highly reactive molecule and in excessive amount it can cause an oxidative stress which causes numerous chronic diseases. The ginseng java plant (Talinum Paniculatum (Jacq) Gaertn) contains various types antioxidants which can neutralize free radicals, such as flavonoid, saponin, alkaloid, tannin, quinone, and provitamin A. Aims: This study aims to analyze the effects of ginseng java roots (Talinum Paniculatum) extract on Malondialdehyde (MDA) levels in male white Sprague Dawley rats with a forced swimming test model. Methods: This study used 30 Sprague Dawley rats which were assigned into 5 different groups, such as the negative control group, positive control group, ginseng roots extract dose of 0.35 mg/200g rats body weight/day group, ginseng roots extract dose of 0.70mg/200g rats body weight/day group, and ginseng roots extract dose of 1.40 mg/200g rats body weight/day group. On the 28th and 35th day the Sprague Dawley rats were treated with a Forced Swimming Test model. The MDA level was measured on the 28th and 35th day using TBARS assay method and statistical analyze by Kruskall Wallis test with p value less than 0.05 was considered significant. Results: This study showed that the administration of ginseng java roots extract dose of 0.35 mg/200g rats body weight/day, dose of 0.70mg/200g rats body weight/day, and dose of 1.40 mg/200g rats body weight/day were significantly lowering the MDA level (p<0.001). In addition, there were significant differences between treatment groups with various doses (p<0.001). Conclusion: The treatment of ginseng java roots (Talinum Paniculatum) extract significantly lowered the MDA level in the Sprague Dawley Rats with Forced Swimming Test model.