Abstract
AbstractCurrent literature suggests that the reliability of results obtained from electrophoretic studies using material which has been frozen is in doubt. Banding patterns of lactate dehydrogenase and non‐specific esterase produced by isoelectric focusing (IEF) of fresh tissues from the goldfish, Carassius auratus, are presented. Changes which occur in these patterns due to repeated freezing and thawing involve a reduction in both the intensity and the number of bands. Differences in the IEF profiles are extensive and consistent. Changes also occur in material which has been frozen for a ten‐week period and thawed once. Storage in liquid nitrogen, ‐180°C, as distinct from a conventional freezer at ‐20°C, produes no significant improvement. Interpretation of results from material that has been frozen must therefore be undertaken with extreme care, especially when sensitive separation techniques like IEF are used.
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