The Effects of Foliar Application of Phenoxy and Imidazoline Family Herbicides on the Limitation of Primary Photosynthetic Processes in Galega orientalis L.

Jaroslav Lang,Barbora Zikmundová,Josef Hájek,Peter Váczi,Miloš Barták

doi:10.3390/agronomy12010096

Jaroslav Lang, Barbora Zikmundová + Show 3 more

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https://doi.org/10.3390/agronomy12010096

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Abstract

Fodder galega (Galega orientalis) is a perennial, wintering plant with great potential for agricultural development. The species has a large yield potential and exceptional adaptability to various environmental conditions. The sensitivity of G. orientalis to herbicides, however, as well as the photosynthetic performance of the species, are generally unknown. Our study aimed to evaluate the effects of the application of selected phenoxy herbicides (MCPA, MCPB) and the imidazoline family herbicide (IMA) on the parameters of primary photosynthetic processes as understood through fast chlorophyll fluorescence kinetics (OJIP). The effect of cultivation temperature was also investigated in the plants grown at 5, 18 and 25 °C. Time courses of OJIP-derived parameters describing photosystem II functioning after foliar application revealed that the plants showed negative responses to the herbicides in the order MCPB–MCPA–IMA within 24 h after the application. The application of herbicides decreased the values of maximum chlorophyll fluorescence (FM) and increased minimum fluorescence (F0), which led to a reduction in the maximal efficiency of PSII (FV/FM). Applications of MCPA and MCPB decreased variable chlorophyll fluorescence at 2 ms (VJ), 30 ms (VI) and VP, as well as the performance index (PIABS), which is considered a vitality proxy. The application increased absorption flux (ABS/RC), trapped energy flux (TRo/RC) and dissipated energy flux (DIo/RC). The effects were more pronounced in plants grown at 18 and 25 °C. The study revealed that the OJIP-derived parameters sensitively reflected an early response of G. orientalis to the foliar application of herbicides. Negative responses of PSII were more apparent in MCPA- and MCPB- exposed plants than IMA-exposed plants.

Highlights

The effects and plant responses to herbicide application depend on the mode of action, dose and plant species [1]
The aim of the study was to evaluate the effects of selected herbicides (MCPA, MCPB and imidazoline family herbicide (IMA)) on primary photosynthetic processes of G. orientalis and to find sensitive, fastresponding chlorophyll fluorescence parameters that could be used for the early indication of MCPA, MCPB- and IMA-caused stress in G. orientalis
MCPA and MCPB caused a substantial decrease in chlorophyll fluorescence signal; this was demonstrated as a “flattening” of the OJIP curves with time of exposure

Summary

Introduction

The effects and plant responses to herbicide application depend on the mode of action, dose and plant species [1]. Negative effects of herbicides on plants have been reviewed and the compounds classified into three functional/action groups [2]: (1) those interacting directly with photosynthesis (inhibitors of photosystem I and II), (2) those inhibiting carotenoid synthesis and (3) those with mechanisms of action generating reactive oxygen species and lipid peroxidation (uncouplers and inhibitors of protoporphyrinogen oxidase). Some studies (e.g., [3]) describe growth-inhibiting herbicides. For plants affected by any of these groups, induced chlorophyll fluorescence is a good biomarker capable of identifying certain herbicide modes and actions. Herbicide resistance to photosystem II inhibitors was reviewed in [4]. Photosystem II inhibitors are further subclassified into numerous chemical classes

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