The effects of five fish anaesthetics on acid–base balance, hematocrit, blood gases, cortisol, and adrenaline in rainbow trout

Abstract

Some physiological aspects of five fish anaesthetics in rainbow trout were investigated. The effects of benzocaine, 2-phenoxyethanol, MS-222 (Sandoz), metomidate, and carbon dioxide gas (CO2) on acid–base regulation, hematocrit, blood gases, and cortisol and adrenaline concentrations were determined in resting rainbow trout fitted with chronic catheters in the dorsal aorta. A severe hypoxia developed with the cessation of breathing in deep anaesthesia. This was accompanied by a rise in blood [Formula: see text] and adrenaline concentration, and a fall in blood pH. Blood bicarbonate concentrations remained unchanged and cortisol concentrations declined with time. There was a transient increase in hematocrit coinciding with the increase in adrenaline concentrations.