Abstract
The expression of SGLT-1 mRNA has been reported in the small intestine of mammals and the rectum of chickens. However, the expression and functional significance of SGLT-1 in bovine rectum is not known. In this study, we studied the effects of fasting and grazing on SGLT-1 gene expression in biopsy epithelial tissue of bovine rectum. In Japanese Black beef cattle, i) SGLT-1 gene expression was measured by quantitative real-time PCR in the biopsy rectal epithelia samples obtained through an endoscope, ii) SGLT-1 gene expression in the rectal epithelial tissues increased at 48 and 72 h after fasting correlating with a decrease in body weight. iii) SGLT-1 gene expression decreased after one month from the start of grazing (May to June) and then stabilized until the end of the grazing period (June to October) in the rectal epithelial tissues of grazing cattle. In conclusion, it is clear that SGLT-1 gene expression in the rectal epithelial tissue is increased by a restricted dietary condition.
Highlights
The expression and functional significance of Sodium dependent glucose transporter-1 (SGLT-1) in bovine rectum is not known
The rectal epithelial tissues were obtained around 40-50 cm from the anus by biopsy forceps using an endoscope
The SGLT-1 gene expression is very low in bovine rectum, we were able to detect the SGLT-1 gene expression in the whole tissue and the biopsy epithelial tissue of bovine rectum
Summary
The expression and functional significance of SGLT-1 in bovine rectum is not known. It is easy to obtain a sample of biopsy epithelial tissue in animals of our institute, which basically conform with the grazing cattle because rectum is the terminus of gastro- guidelines for the care and use of laboratory animals of the intestinal tract. The influence of grazing period on the changes in SGLT-1 gene expression in the biopsy rectal epithelia is not known.
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