The effects of Factor II (rs1799963) polymorphism on recurrent pregnancy loss in Iranian Azeri women

Abstract

Recurrent pregnancy loss (RPL) is a heterogeneous condition consisting of three or more consecutive abortions before the 20 weeks of gestation. Despite extensive research to explain the causative effects of RPL, about 50–60% of RPLs are still idiopathic. Despite the increasing prospective studies with sufficient power related to the association between various thrombophilias and RPL, controversy still remains regarding screening for thrombophilia in women with RPL. The objective of this study was investigate the association between RPL and polymorphism in Factor II G20210A gene in Iranian Azeri women. The subjects in this study consisted of 320 women with RPL and 320 healthy women from Iranian Azeri population. After genomic DNA extraction from blood samples, genotyping was performed by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) method. The obtained data were analyzed using statistical software. No significant association was indicated between Factor II G20210A polymorphism and RPL ( $P>0.05$ ). The genotype frequencies of F-II in the case group were 98/12% GG, 1/88% GA and 0% AA, while the frequencies in the control group were 98/43% GG, 1/56% GA and 0% AA, respectively. There is no significant association between Factor II G20210A (rs1799963) polymorphism and RPL among Iranian Azeri women.