The effects of extract ofWujia-Shenghua capsule on proliferation of the MCF-7 cells

Abstract

ObjectiveTo observe the effect of estrogenic-like effects ofWujia-Shenghua capsule and effective medication site.MethodsThrough D-101 macroporous resin column methods,Wujia-Shenghua capsule with 60% ethanol extraction was separated for water elution part, 20% ethanol elution part, 40% ethanol elution part, 60% ethanol elution part. Then each elution part was respectively mixed into concentration of 10mg/ml,1mg/ml, 0.1mg/ml, 0.01 mg/ml and acted on the MCF-7 cell to have, MTT test and rate of PR calculation.Results Compared with the blank control group（100%）, when the concentration was 0.1 mg/ml, water elution part, 20% ethanol elution part and 60% ethanol elution part（98.10%, 101.06%, 106.04%）had no effect on the proliferation of MCF-7 cells, there was not statistically significant（P〉0.05）, while the 40% ethanol elution part（108.22%）can promote the proliferation of MCF-7 cells,there was a statistically significant（P〈0.05）. When the concentration was 1 mg/ml, 20%, 40% and 60% ethanol elution part（111.72%, 122.48%, 115.35%）can distinctly promote the proliferation of MCF-7 cells, there was a significant difference between four groups（P〈0.01）.ConclusionThe 40% ethanol elution part ofWujia-shenghua capsule has the strongest estrogen activity on plant. Key words: Wujia-Shenghua capsule extract MCF-7 cell MTT test Plant estrogen-like effects Effective medication site