The effects of enrofloxacin exposure on responses to oxidative stress, intestinal structure and intestinal microbiome community of largemouth bass (Micropterus salmoides)

Abstract

Antibiotic residues in the aquaculture environments may lead to antibiotic resistance, and potentially exert adverse effects on health of the non-target organisms and humans. In order to evaluate the effect of enrofloxacin of environmental concentrations on largemouth bass (Micropterus salmoides). Two hundred and seventy largemouth basses (with an average weight of 7.88 ± 0.60 g) were randomly divided into three groups, and separately exposed to 0, 1, 100 μg/L enrofloxacin (Control, ENR1, ENR100) for 30 days to detect the effect of enrofloxacin on the growth performance, oxidative stress, intestinal microbiota structure, inflammatory response and structure of the intestine. The results showed that ENR significantly reduced the final body weight (FBW) and weight gain rate (WGR), and increased feed conversion ratio (FCR) (P < 0.05). The histopathological analysis revealed that the villus width and muscular thickness of anterior intestine were significantly decreased with the increasing of enrofloxacin concentration. The activity of SOD was significantly increased at enrofloxacin stress, while CAT and POD activity were significantly decreased compared to control group (P < 0.05). The activities of lysozyme (LZM), alkaline phosphatase (AKP) and peroxidase (POD) in ENR1 was higher than that of control and ENR100 groups. Enrofloxacin treatment up-regulated the expression IL-1β and TNF-α, and down-regulated IL-10, and decreasing the expression level ZO-1, claudin-1, and occludin. Furthermore, the enrofloxacin treatment significantly decreased the intestinal bacterial diversity (P < 0.05). Exposure to 100 μg/L enrofloxacin obviously increased the relative abundance of Bacteroidota, Myxococcota, and Zixibacteria of fish gut, and reduced Firmicutes; 1 μg/L enrofloxacin considerably increased Bacteroidota, Myxococcota, and Actinobacteria, and reduced Firmicutes. The relative abundance of DTB120 and Elusimicrobiota was positively correlated with the occludin and claudin-1 gene. Taken together, exposure to enrofloxacin inhibited the growth of largemouth bass, influenced intestinal health, and induced dysbiosis of the intestinal microbiota.