The Effects of Different Oxytetracycline and Copper Treatments on the Performance of Anaerobic Digesters and the Dynamics of Bacterial Communities

Yun Zhang,Guangcai Zhang,Xin Ke,Xiaodan Gao,Wei Sun,Haijun Zhang,Weiyun Wang

doi:10.1155/2018/1897280

Abstract

Oxytetracycline and copper are the common residues in animal manures. Meanwhile, anaerobic digestion is considered as a clean biotechnology for the disposal of animal manures. In this paper, the performance of anaerobic digesters and the dynamics of bacterial communities under the different treatments of oxytetracycline and copper were discussed. The parameters of methane production and pH values were studied to reflect the performance of anaerobic digester. Results showed that the changes of methane production and pH values were not obvious compared with the control. This means that the treatments of oxytetracycline and copper almost have no effects on the performance of anaerobic digesters. This phenomenon might be due to the chelation reaction between oxytetracycline and copper. This chelation reaction might reduce the toxicity of oxytetracycline. The study on the dynamics of bacterial communities was based on the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method. Results indicated that the bacterial communities had significant differences under the different treatments of oxytetracycline and copper. Uncultured Bacteroidetes bacterium (CU922272.1) and uncultured Bacteroidetes bacterium (AB780945.1) showed adaptability to the different treatments of oxytetracycline and copper and were the dominant bacterial communities.

Highlights

IntroductionWith the development of livestock, the residual antibiotics and heavy metals in animal manure have attracted more and more world-wide attention
With the development of livestock, the residual antibiotics and heavy metals in animal manure have attracted more and more world-wide attention. This residual phenomenon is due to the low bioavailability of antibiotics and heavy metals which are widely added to the feeds in order to control diseases and enhance the growth of livestock animals [1,2,3,4]
Previous studies had found that oxytetracycline had inhibition on methane production during the anaerobic digestion [28, 29]

Summary

Introduction

With the development of livestock, the residual antibiotics and heavy metals in animal manure have attracted more and more world-wide attention. This residual phenomenon is due to the low bioavailability of antibiotics and heavy metals which are widely added to the feeds in order to control diseases and enhance the growth of livestock animals [1,2,3,4]. The residual metals in manure were considerable [6, 7]. Oxytetracycline and copper are the common residues in animal manures. It was found that the residual oxytetracycline in cow manure ranged from 0.32 mg/kg to 59.59 mg/kg [8]

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Conclusion