The effects of different concentrations of nano silver on elimination of bacterial contaminations and stimulation of morphogenesis of Sorbonne lily in vitro culture

Abstract

Nano silver (NS) have been known as a novel material which cannot only destroy the structure of cell microorganism but also have positive effects on in vitro plant morphology. In this research, NS was used to sterilize lily samples and stimulate lily morphogenesis in vitro. Separated bulb scales were treated in solutions of NS with four different concentrations (100, 200, 300 and 400 ppm) in five different times (60, 90, 120, 150 and 180 min) that were compared with controls being soaked in sodium hypochlorite solution. Besides, four different concentrations (2, 4, 6, and 8 ppm) of NS were also added to culturing medium that aimed to test effects of NS on lily plant growth parameters in vitro. The result illustrates that NS with 300 ppm concentration was the most useful solution for sterilizing the lily samples in 120 min., making up 87.78% sample survival and clean. Moreover, NS enhanced bulblet formation from bulb scales samples. On the medium supplemented with 4 ppm NS, 100% bulb scales were formed new bulblets with a coefficient, 2.98 after six weeks culturing; and on the rooting medium, 6 ppm NS made up the highest percentage of bulblets samples rooting, 87.33%, with the highest root number per bulblet, 3.67 and average 2 cm in length, each.