Abstract
BackgroundRecurrent spontaneous abortion (RSA) is a serious problem in pregnancy. The exact etiology of RSA is unknown in more than 50% of all the patients. However, genetic variations are known as susceptibility factors for idiopathic RSA. Considering the role of miRNA biosynthesis machinery in the miRNA production and effect of miRNAs on various diseases, this study aimed to evaluate the effects of DICER1 rs3742330 and DROSHA rs6877842 polymorphisms on RSA risk.MethodsIn this case‐control study, 150 RSA patients and 195 age‐matched healthy female controls were recruited. Both polymorphisms were genotyped using PCR‐RFLP method.ResultsThe frequency of DICER1 rs3742330AG genotype was higher in the control group (P = .022). There was a statistically significant association between rs3742330 polymorphism and a reduced RSA risk in dominant and allelic models (P = .013 and P = .007, respectively). No statistically significant association was found between DROSHA rs6877842 variant and RSA risk. The combination of AG and GC genotypes and G‐G alleles of DICER1 rs3742330 and DROSHA rs6877842 polymorphisms led to a decreased RSA risk. However, the synergic effect of rs3742330A and rs6877842G alleles (A‐G) and AA‐GG genotypes was associated with an increased RSA risk.Conclusionthe DICER1 rs3742330AG genotype and combination of AG and GC genotypes and G‐G alleles of DICER1 rs3742330 and DROSHA rs6877842 polymorphisms were associated with a reduced RSA risk.
Highlights
Recurrent spontaneous abortion (RSA) is defined as the occurrence of two or more spontaneous abortions before 20th week of gestation
When we analyzed the effects of the combination of DICER1 rs3742330 and DROSHA rs6877842 polymorphisms on RSA suscep‐ tibility, we found higher frequencies of AA/GG combined genotypes in RSA cases and all other combined genotypes in controls (P = .008, Table 3)
Aberrant expression and dysregulated pregnancy associated with miRNAs have been proven in several pregnancy complications, in‐ cluding preeclampsia and RSA.[25,26]
Summary
Recurrent spontaneous abortion (RSA) is defined as the occurrence of two or more spontaneous abortions before 20th week of gestation. Methods: In this case‐control study, 150 RSA patients and 195 age‐matched healthy female controls were recruited. Both polymorphisms were genotyped using PCR‐ RFLP method. There was a statistically significant association between rs3742330 polymorphism and a reduced RSA risk in dominant and allelic models (P = .013 and P = .007, respectively). The combination of AG and GC genotypes and G‐G alleles of DICER1 rs3742330 and DROSHA rs6877842 polymorphisms led to a decreased RSA risk. Conclusion: the DICER1 rs3742330AG genotype and combination of AG and GC genotypes and G‐G alleles of DICER1 rs3742330 and DROSHA rs6877842 polymor‐ phisms were associated with a reduced RSA risk
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