The Effects of Cortisol on the Binucleate Cell Population in the Ovine Placenta During Late Gestation

Abstract

Large granulated binucleate cells (BNCs) producing placental lactogen (PL) and pregnancy-associated glycoproteins (PAG) are present in all ruminant placentae throughout pregnancy. These BNC account for 15–20% of the cells in ovine trophectoderm for most of gestation but decrease in number close to term at the same time that fetal cortisol levels rise. The present study investigated the effects of cortisol on the BNC population using immunohistochemistry to count BNCs in ovine placentomes during late gestation and after experimental manipulation of the fetal cortisol level by fetal adrenalectomy and exogenous cortisol infusion. Abolition of the prepartum rise in fetal cortisol prevented the normal decline in BNC numbers towards term. Conversely, raising cortisol levels in immature fetuses to prepartum values prematurely reduced placental BNC numbers. However, a small population of BNC remained, even at the highest cortisol concentrations. When all the data were combined irrespective of treatment or gestational age, there was a significant inverse correlation between fetal plasma cortisol and the number of BNCs in the ovine placenta. These findings show that cortisol regulates the BNC population in ovine placenta during late gestation. They also have important implications for the production of PL and PAG during ovine pregnancy.