The effects of confinement stress on circulating prolactin levels in rainbow trout (Oncorhynchus mykiss) in fresh water

Abstract

Rainbow trout were confined for 48 hr, during which time water quality either was allowed to deteriorate (resulting in elevated NH3, elevated free CO2, and reduced dissolved O2) or was maintained at preconfinement levels. Fish were removed and. blood samples taken at 0, 2, 4, 8, 24, and 48 hr after the onset of confinement from both stressed (confined) and unstressed (unconfined) fish. Plasma cortisol and plasma prolactin (PRL) levels were determined using specific RIAs. Chronic confinement of rainbow trout, accompanied by a decline in water quality, resulted in significant elevation of plasma cortisol, maintained for the period of confinement. Plasma PRL levels were significantly lower in stressed fish, by up to 60% relative to control fish, during the first 24 hr of confinement. The stress of confinement alone, in the absence of deterioration in water quality, produced similar results, with the change in prolactin levels being less rapid but more prolonged under these conditions.