The effects of chronic exposure of manganese on antioxidant enzymes in different regions of rat brain

Abstract

The present study was designed to investigate if chronic exposure to manganese (Mn) produces an effect on antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) activities and reduced glutathione (GSH) content in different regions of rat brain. Adult male Sprague-Dawley (CD) rats were dosed with 0, 2.5 or 5.0 mg MnCl2/kg, for 3 months (5 days/week). The activity of total superoxide dismutase did not vary significantly in any region of the brain with either 2.5 or 5.0 mg MnCl2/kg. A significant increase of Mn-superoxide dismutase (Mn-SOD) activity was attained in hippocampus, cerebellum and brain stem. The Cu, Zn-superoxide dismutase activity was reduced in all regions of the brain, however, reduction was not statistically significant. No significant effect of Mn on glutathione peroxidase activity was observed in any region of the brain. Glutathione content was significantly reduced in cerebellum, whereas, no change was observed in other brain regions. The results show that chronic exposure to manganese significantly increased the Mn-superoxide dismutase activity in selected brain regions. Therefore, increased Mn-SOD may enhance the antioxidant ability of the brain to reduce oxidative stress. © 1997 John Wiley & Sons, Ltd.