Abstract
ABSTRACTThe effects of Alternaria alternata (Fr.) Keissler, yeast extract, cellulase, jasmonic acid (JA), hydrogen peroxide (H2O2), and salicylic acid (SA) on the capsidiol accumulation were investigated in the cell suspension cultures of three genotypes (resistant, PM 217 and PM 702; susceptible 11 B 14) of Capsicum annuum L. which have different resistant to Phytophthora capsici Leon. Cell suspension cultures were obtained from hypocotyle expiants of pepper seedlings emerged under in vitro conditions. The two-week old green pepper suspension cultures were elicited using auto-claved hyphae suspension of A. alternata, yeast extract, cellulase, JA, H2O2 and SA elicitors. The capsidiol concentration was determined by gas chromatograhy analysis, 24 and 48 h after elicitation. The amounts of capsidiol accumulated in the cultures with elicited cellulase and A. alternata were higher than the other elicitors. Besides a negligible amount of capsidiol accumulation was observed with H2O2 and nothing with SA in the concentration range studied. Maximum capsidiol concentration was obtained as 91.42± 3.9 mg/mL in the cultures of 11 B 14 susceptible genotype elicited with 1500 mg/mL cellulase at 24 h later. The capsidiol concentrations were high for resistant genotypes (PM 217 and PM 702) in the cultures with elicited A. alternata and yeast extract. But the capsidiol concentrations were high for susceptible genotype(11 B 14) in the cultures with elicited cellulase, JA, H2O2 and SA.
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