The effects of aspirin DL-lysine and sodium salicylate on dentin formation in the rat incisor.

Abstract

The effects of aspirin DL-lysine (Venopirin) and sodium salicylate on dentin formation in the rat incisor have been studied by a time marking method using lead salt and by the autoradiography using[3H]-proline as a collagen precursor. The rates of dentin formation in both the maxillary and mandibular incisors were significantly inhibited after the intravenous injection of both drugs. The inhibitory effect was found to be dose-dependent. There was no significant difference between the inhibitory potencies of both drugs at each dose in a range from 0.5 to 3.0mmol/kg. The number of autoradiographic silver grains over both the odontoblastic layer and the dentinal matrix decreased after the injection of both drugs. However, the percentage of silver grains over the dentinal matrix in relation to the total number of silver grains was relatively constant. A radiolucent zone which appeared in the contact microradiograph of the experimental group showed that the mineralization of dentin had been inhibited by both drugs. The results of high performance liquid chromatography confirmed that aspirin rapidly converted to salicylate in blood plasma. These results suggest that both Venopirin and sodium salicylate inhibit dentin formation through the mechanism which reduces the incorporation of collagen precursors into odontoblasts. It is also suggested that the effects of both drugs on dentin formation is mainly due to salicylate.