The Effects of Artificial Night Lighting on Tail Regeneration and Prey Consumption in a Nocturnal Salamander (Plethodon cinereus) and on the Behavior of Fruit Fly Prey (Drosophila virilis).

Abstract

Simple SummaryAmphibians, including salamanders, are declining worldwide at an alarming rate due to a variety of factors that include habitat decline and destruction and environmental pollutants. Artificial light at night (ALAN) due to human activities is a nearly ubiquitous pollutant and can have serious consequences for amphibians. We examined the impact of ecologically-relevant levels of ALAN on tail regeneration in the eastern red-backed salamander, prey consumption by these salamanders and behavior of their fruit fly prey. We found that ALAN reduced the rate of salamander tail regeneration at some light levels above the naturally dark nocturnal illumination and increased the activity of their prey but not always in a simple, linear fashion. Thus, ALAN, even at very low levels, can influence the physiology and regeneration of a nocturnal salamander.As human development continues to encroach into natural habitats, artificial light at night (ALAN) has increasingly become a concern for wildlife. Nocturnal animals are especially vulnerable to ALAN, as the physiology and behavior of nocturnal species have evolved under conditions associated with predictably dark environments. Studies exposing amphibians to constant bright light provide evidence for changes to normal metabolism, growth, and behavior, but few of these studies have used treatments of dim ALAN comparable to that found in affected habitats. Eastern red-backed salamanders, Plethodon cinereus, use their tails for fat storage and communication, are capable of tail autotomy as an antipredator mechanism, and can regenerate the tail in its entirety. We examined the effect of different, ecologically-relevant intensities of ALAN on the rate of tail regeneration in adult P. cinereus. We hypothesized that ALAN would increase tail regeneration rates such that salamanders exposed to higher levels of light at night would regenerate tails faster than those exposed to lower light levels. In a controlled laboratory setting, we exposed salamanders (N = 76) in test chambers to nocturnal illuminations of 0.0001 lx (no ALAN, natural nocturnal illumination dark control), 0.01 lx (weak ALAN), 1 lx (moderate ALAN), or 100 lx (bright ALAN, equal to dim daytime and our day lighting treatment) for a period of 90 d immediately following tail autotomy. In addition, because these salamanders eat mostly live, moving prey, we investigated the impact of ALAN on the behavior of prey (Drosophila virilis) fed to the salamanders in our laboratory trials, which could alter feeding and regeneration rates in salamanders. We predicted that prey consumption would not be affected by ALAN and measured both prey consumption and prey behavior (activity) to examine the potential influence on regeneration. For tail regeneration, we found a non-monotonic response to ALAN, with salamanders exposed to nocturnal illuminations 0.1 lx and 100 lx regenerating tails significantly slower than salamanders in the 0.0001 lx or 1 lx treatments. Prey consumption did not differ among light treatments; however, fruit fly activity increased with increasing ALAN. These results suggest that ALAN influences regeneration rates, but the rate of regeneration is not dose-dependent and is not explained easily by prey consumption or movement of prey. We suggest that tail regeneration in these salamanders may involve a complex mechanism of altered gene expression and/or modulation of hormonal activity (corticosterone, melatonin, serotonin, and/or prolactin) at different intensities of nocturnal lighting.