The effects of aqueous extraction Rosmarinus officinalis L. on rat bladder contractions in vitro

Abstract

Objective. To evaluate the effect of Rosmarinus officinalis L (Labiatae ), an effective and herbal reputed plant for the treatment of urinary ailments, on rat bladder contractilityMethodAnimal experiments were performed in accordance with the national guidelines for use and care of laboratory animals and approved by the local animal care committee of Ataturk University. Urinary bladders were removed from adult male Sprague ‐ Dawley rats. Strips were cut from the bladder body and placed in organ baths containing Krebs solution. The effects of four different doses (150, 300, 600 and 1200 μg/ml) of Rosmarinus officinalis L were investigated on rat bladder contractility induced by 60 mmol/L KCl and 10−4 mol acetylcholine.ResultsContractile tensions were normalized by using the percentage contraction induced by 60 mmol/L KCl and 10−4 mol acetylcholine. The effect of Rosmarinus officinalis L on the urinary bladder contractility induced Ach and KCl. There is no statistical difference between groups (n=6, P>;0.05)ConclusionAny effect of Rosmarinus officinalis L was not observed on the contractions of the urinary bladder of rat that are induced by both Ach and KCl. It can be expressed that Rosmarinus officinalis L which is used in the herbal treatment of some urinary disease does not affect the activity of smooth muscles of urinary bladder.This study was supported by Ataturk University Research Foundation