Abstract
THE EFFECTS OF ALKALI METAL IONS ON THE ACETATE ACTIVATING ENZYME SYSTEM
Highlights
Similar results are obtained with ammonium ion
It is nom apparent that t’he conversion of acetate to the high energy intermediate acetyl CoA (10) can be classified with those enzymatic processes shown to be stimulated by potassium ion and inhibited by sodium ion; e.g., pyruvic phosphopherase (II), phosphotransacetylase (12), and yeast aldehyde dehydrogenase (13)
The marked inhibition of AAE activity by low concentrations of sodium ion and maximal stimulation of activity by potassium ion within the range of physiological intracellular concentrations is of considerable interest
Summary
Preparation of Enzyme-During the progress of this work AAE was prepared in three ways: (a) from a pig heart homogenate by an unpublished New York. T Fellow of the Helen Hay Whitney Foundation, CoA, coenzyme A; AMP, adenosine&phosphate; PPr, inorganic pyrophosphate; Tham, tris(hydroxymethyl)aminomethane; GSH, glutathione. The extract after centrifugation contained a very active AAE and sufficient condensing enzyme to produce citrate at a rapid rate from acetyl CoA and oxalacetate. When AAE was prepared by ammonium sulfate fractionation, the final preparations were dialyzed against Tham (0.02 M, pH 7.5) until free of ammonium ion
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
