Abstract

Glucocorticoid receptor concentration (GR) was determined in mononuclear (MNS) and polymorphonuclear (PMNS) cells isolated from 8 aged and 9 young male dogs. In addition, leukocyte responses to dexamethasone (0.1 mg kg −1 iv) and plasma glucose concentration were also measured. The GR in MNS and PMNS was higher ( p<0.05) in young dogs (6.64±0.57 and 7.04±0.29 fmoles×10 6 cells, respectively, versus 4.40±0.24 and 5.06±0.33 fmoles×10 6 cells, respectively, in aged dogs). The maximum increase in neutrophils (Δ NEU) was lower ( p<0.05) in aged dogs 6003.38±1398.5×10 6 versus 11168.67±1863.16×10 6 cells l −1 in young dogs. The maximum decrease in lymphocytes (Δ LYM) was lower ( p<0.05) in aged dogs 550±56.75×10 6 cells l −1 versus 1825.89±313.1×10 6 cells l −1 in young dogs. In young dogs, significant ( p<0.05) correlations between GR in PMNS and Δ NEU ( r=0.80) and between GR in MNS and Δ LYM ( r=0.76) were observed. In aged dogs, these correlations were not significant. The lower GR value and the lack of correlation between this parameter and its respective Δ in aged dogs suggest that changes in leukocytes responsiveness to glucocorticoids is occurring during the aging process.

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