The effects of adrenergic and cholinergic agents on progesterone production by human corpus luteum in vitro

Abstract

We investigated the effects of adrenergic and cholinergic agents on human corpus luteum production of progesterone in vitro. Luteinizing hormone (LH) (50 ng/ml), dibutyryl cyclic adenosine monophosphate (Bu2cAMP) (10−3M), and prostaglandin E2 (PGE2) (1 μg/ml) significantly stimulated the production of progesterone in short-term (4-hour) cell suspensions of five early and middle luteal phase corpora lutea. The adrenergic agents isoproterenol, norepinephrine, and the cholinergic agents acetylcholine and carbachol at concentrations up to 10−4M did not alter basal or stimulated production of progesterone. Similarly, in long-term (10-day) monolayer cultures of cells from four corpora lutea, human chorionic gonadotropin (hCG) (50 ng/ml) and PGE2 stimulated, but none of the adrenergic or cholinergic agents altered, the production of progesterone significantly, except for an inhibitory effect of norepinephrine and carbachol in the presence of 17β-estradiol (10−7M) added to the culture medium. These results differ strikingly from the consistent stimulatory effect of β-adrenergic agents on the luteal production of progesterone in several animal species.