The Effects of a Novel Magnesium Complex on the Intestinal Magnesium Transporters in HFD Rats

Abstract

Abstract Objectives Magnesium (Mg) is absorbed mainly in the intestine and regulates many physiological processes and signaling pathways. TRPM6, a member of the transient receptor potential melastatin-related (TRPM) protein family, is essential for maintaining the organismal Mg2 + balance in the intestine. This study was done to compare the effects of a new form of magnesium, Mg-N21, to magnesium oxide (MgO) on the expression of intestinal Mg transporters in rats Methods Forty-two male Wistar rats (age: 8-week) were randomly divided into the following groups (n = 7 per group): 1) Control; 2) MgO; 3) Mg-N21; 4) HFD (high fat diet); 5) HFD + MgO; 6) HFD + Mg-N21. Magnesium was dosed at 500 milligrams of elemental Mg/kg diet. All rats were supplemented for 8 weeks. Results HFD intake showed a trend toward decreased ileum TRPM6 expression in rats. However, Mg treatment increased ileum TRPM6 expression in HFD rats. TRPM7 expression was not significantly changed. In addition, claudins (Cldn-12 and −15) expression decreased in the ileum of rats fed a HFD. The expressions of ileum claudins were enhanced in Mg-treated rats. The effectiveness of Mg as Mg-21 was more pronounced than Mg as MgO, which can be associated with higher bioavailability. Conclusions Results of the present study show that Mg-N21 is more protective than magnesium oxide on the Mg transporters in rats fed HFD. Funding Sources JDS Therapeutics, LLC Turkish Academy of Sciences.