Abstract
To investigate the properties of the γ-aminobutyric acid (GABA)-synthesizing enzyme, glutamate decarboxylase (GAD), in the pancreatic islets and brain of the rat, GABA concentration in the pancreatic islets and brain was measured after administration of 3-mercaptopropionic acid (3-MP) in in-vivo and in-vitro studies. Fifteen and sixty minutes after administration of 3-MP at 25 mg kg−1 intraperitoneally, GABA concentration in the pancreatic islets decreased by 30–35% and GABA concentration in the hypothalamus, the superior colliculus and the hippocampus of the brain decreased by 25–30%. GABA concentration in the pancreatic isolated islets in-vitro was measured after incubation of 3-MP in the range 10−7-10−4 M for 60 min. GABA concentrations in the pancreatic islets decreased on stimulation by 3-MP in a dose-dependent manner. The activities of GAD in the pancreatic islets can be modified by a convulsant, in this case 3-MP, in-vivo and in-vitro. These results suggest that the properties of GAD may be similar in the pancreatic islets and brain.
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