The effects of α2-adrenoceptor stimulation on neocortical EEG activity in control and 6-hydroxydopamine dorsal noradrenergic bundle-lesioned rats

Abstract

The present study investigated the effects of a α 2-adrenoceptor agonist, D-medetomidine (0.3, 3.0, 30.0 and 300.0 μg/kg, s.c.),on neocortical EEG activity in control and 6-hydroxydopamine dorsal noradrenergic bundle-lesioned rats. D-Medetomidine at 0.3, 3.0 and 30.0 μg/kg dose dependently increased waking-immobility-related high-voltage spike and wave spindles. Movement and waking-immobility-related slow wave activity was increased at doses of 3.0, 30.0 and 300.0 μg/kg. D-Medetomidine at 300.0 μg/kg produced continuous 1–2 Hz slow wave activity and the animals were markedly sedated. In rats injected with D-medetomidine at 0.3, 3.0 and 30.0, μg/kg EEG activity could be desynchronized (block of high-voltage spindles and slow waves) by pinching the tail. However, rats injected with D-medetomidine at 300.0 μg/kg showed no change in EEG activity or behavior following tail pinching. D-Medetomidine induced similar EEG activity (high-voltage spindles and slow waves) and behavioral changes (sedation) in 6-hydroxydopamine dorsal noradrenergic bundle-lesioned rats. Atipamezole, an α 2-adrenoceptor antagonist, blocked D-medetomidine-incuded EEG and behavioral changes in control and 6-hydroxydopamine dorsal noradrenergic bundle-lesioned rats. Based on the present results we suggest that stimulation of presynaptic noradrenergic fibers is not a prerequisite for the increase of high-voltage spindle and slow wave activity induced by an α 2-adrenoceptor agonist and that the magnitude of EEG slowing induced by D-medetomidine correlates with the decreased behavioral response to sensory stimulation.