Abstract
1. 2,4-Dinitrophenol and five other uncoupling agents (at concentrations of 1.0 mM) inhibit the utilization of nitrate by about 80–95%. The inhibitions can be relieved by increasing nitrate concentrations. 2. The uncoupling agents fail to inhibit the activity of nitrate reductase in cell-free extract. 3. Inhibition of nitrite utilization by the six uncoupling agents depends in the experimental conditions. Under heterotrophic conditions, all six inhibit. When cultures are illuminated (particularly in the absence of CO 2) three of the uncouplers are much less effective as inhibitors than they are under heterotrophic conditions.
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